Frequently Asked Questions

Got questions? This page offers a list of frequently asked questions where you may be able to find answers. If you can’t find the answer you're looking for, please drop us an email at info@nemalifeinc.com with your question and we’ll get back to you.

Worm Strains

Do I need to grow animals on plates to do lifespan assays in the Infinity System?


Yes. Animals are grown to young adulthood on agar plates using standard protocols (e.g. as described in Wormbook). Animals at young adulthood (typically older than day 2 from hatching) are transferred into Infinity Chip for life-long culture.




Can I grow animals in liquid culture and then transfer to Infinity Chips?


Yes. However, we don’t recommend it since long-term culture in liquid media disrupts normal development and decreases growth rates within the population. If you really need to, it is best to limit the animals in liquid culture to a single generation.




What is the optimal age and size of animals to be introduced used in the Infinity System?


The Infinity system has been optimized for wild-type animals grown at 20 degrees celsius that are loaded approximately 65 hours after age synchronization.




Can animals of smaller size be used in the Infinity System?


Given that strains and mutants might go through developmental stages at different times depending on growth conditions, the Infinity System can be used with strains and mutants whose body diameter is more than 50 microns. Larger animals are preferrable so as to prevent sample loss during washing and feeding in the Infinity chips.




What worm strains could be used in the Infinity System?


Any worm strain that has a body diameter of more than 50 microns can be used.





Infinity Chips

What medium can I use for culturing the worms in the Infinity Chips?


We recommend liquid NGM. Other culture media (e.g. M9 or S-buffer) compatible with nematode growth can also be used.




How many worms can be loaded with the Infinity Chips?


We recommend 60 -70 worms per chip. If more worms are needed for an experiment, we recommend using multiple chips for the same condition.




Can the animals survive in the Infinity Chip for longer than 24 hours without bacterial food?


Yes. However, we recommend that food is replenished daily unless you are interested in studying effects of starvation.




Is there bagging in the Infinity Chips?


The frequency of bagging in wild-type animals is similar to that of animals cultured on agar plates.




How many times can I reuse the Infinity Chips?


We recommend that the Infinity Chips be used only once for life-long experiments since there could be significant accumulation of chemicals (e.g. drugs, metabolites, pheromones) in the chamber thereby altering the culture environment for subsequent use.





Bacterial Food

What type of bacterial food can be used in the system?


All types of microbial food can be used. Any food that does not clog the Infinity culture chambers or form biofilms can be used.




What is the bacterial concentration used for daily feeding of animals?


We recommend 20 mg/mL of final bacterial concentration in media for culturing animals in the Infinity Chips.




What is the protocol for preparing bacterial food?


We grow standard overnight cultures of E. coli OP50. We pellet the bacterial cells by centrifugation at 3500 – 4000 RPM for 10 minutes and resuspend the cells in liquid NGM to the desired concentration.




How long can I store the prepared bacterial food?


At 4 degrees celsius, bacterial food can be stored for up to 2 weeks. Also, bacterial food can be stored frozen at –20 degrees celsius for up to 3 months.




Can RNAi bacteria be fed in the Infinity System?


Yes. RNAi bacteria can be prepared using Wormbook protocols and fed to the worms.





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