Unleash the power of whole-life screening and data-driven intelligence
Whole-life studies with C. elegans are traditionally performed on agar-plates. This approach is time consuming due to animal transfers and manual scoring of animal response, and therefore, does not scale favorably for high throughput screening. At NemaLife, we have developed the world’s first benchtop screening system that overcomes current challenges in conducting large-scale in vivo assays for applications in genetic and
drug screens, toxicology, and mechanistic studies.
The Infinity Desktop Screening System
The workflow involves loading adult animals in the Infinity chip, followed by washing, feeding and imaging using the Infinity processor, and finally data analysis with the Infinity code.
The Infinity chip is a disposable microfluidic chamber that is engineered to create a soil-like environment where C. elegans crawl with plate-like behaviors.
Key benefits of this chip are:
Maintenance of life-long synchronized adult populations without using progeny-blocking drugs
Animals are fed, and progeny are removed and collected at defined time-intervals
Drugs, stressors and RNAi can be added at any time to the population
Animals can be immobilized with anesthetics and pluronic gel
Optical transparency of the chamber allows brightfield, darkfield, fluorescence and confocal imaging
The Infinity processor is a benchtop machine that controls fluid flow to the Infinity chip and enables image acquisition of the synchronized animal population.
Key features of this system are:
Two chips can be processed at a time on the machine
Programmable regimens of feeding and reagent addition
Collection of progeny for downstream analysis and multi-generational studies
Time-lapse imaging can be performed with images directly saved to the cloud
Infinity code is a video analysis software that detects and tracks animals.
Current features of the software include:
Batches of hundreds of videos can be processed in one go
Capability of annotation to correct for false positives
Readouts such as live animal counts and body sizes
More features to be added soon